Part:BBa_K3657031
MS2 Coat Protein coupled with C-terminal GFP
This is a fusion protein containing MS2 Coat Protein (BBa_K3218006) and C-terminal Split-GFP (BBa_K3657028).
Usage and Biology
MS2 Coat Protein binds specifically the MS2 RNA hairpin (BBa_K3113006).
C-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of N-terminal Split-GFP (BBa_K3657027).
This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the N-terminal of the Split-GFP planned for the experiment: BBa_K3657030. You can find out more about the planned experiment here: iGEM Heidelberg 2020.
Due to the COVID19 pandemics we did not obtain empirical data about this part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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